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. 2011 Jul 1;39(18):7931–7945. doi: 10.1093/nar/gkr523

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Restoration of H3K56 acetylation is regulated by ATM checkpoint kinase. (A) AT cells are deficient in the restoration of H3K56 acetylation after UV repair. OSU-2 and AT cells were irradiated with 5 and 10 J m⁻² UV, allowed to repair for 4 and 48 h and harvested to prepare whole-cell lysates. H3K56 acetylation levels were determined by western blotting. (B) AT cells are deficient in the restoration of H3K56 acetylation, but not the deacetylation in response to UV irradiation. AT cells were irradiated with 10 J m⁻² UV and cells harvested at various intervals of post-UV repair. Whole-cell lysates were prepared and H3K56Ac levels analyzed by western blotting. (C) Depletion of ATM results in defective restoration of H3K56 acetylation. HeLa cells stably transfected with ATM shRNA were irradiated with 10 J m⁻² UV, allowed to repair and harvested at various times. Whole-cell lysates were prepared and H3K56Ac levels determined by western blotting.