Figure 4.

Characterization of loose heteroduplexes by fluorescence anisotropy. 5′ 5/6-Rh6G mini-TAR RNA was mixed with increased amounts of mini-cTARS2 (triangles) or mini-cTARS3 (squares) in the absence of NC and incubated at 4°C for 60 min as described in ‘Materials and Methods’ section. The fluorescence anisotropy of the mixture was measured at the end of incubation.