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. 2001 Apr 10;98(8):4449–4454. doi: 10.1073/pnas.071547598

Figure 3.

Figure 3

JSRV env transcripts. (A) Northern blotting of total RNA collected from 293T cells transfected with pCMV2JS21, pCMV3JS21ΔGP, and mock-transfected 293T cells. Filters were hybridized with the env-2 probe as described in Materials and Methods. 293T cells transfected with pCMV3JS21ΔGP lack the 7.5-kb band (corresponding to the full-length JSRV genome) because the gag and pol sequences have been deleted. (B) Northern blotting of total RNA from NIH 3T3 cells transformed by pCMV2JS21, parental NIH 3T3 cells, lung from a healthy sheep, and lung from tumor lesions of two sheep affected by OPC. Both the 7.5- and 2.4-kb transcripts are readily visible whereas the 1.2-kb transcript is present at a low level.