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. 2001 Apr 10;98(8):4449–4454. doi: 10.1073/pnas.071547598

Figure 4.

Figure 4

Identification of JSRV env transcripts. (A) Northern blotting on 293T cells transfected with pCMV2JS21 vs. mock-transfected cells was carried out to identify the structure of the 1.2-kb env transcript. Blot hybridization was carried out in parallel with a U3 probe and with the env-2 and env-upstream probes. The 1.2-kb transcript is apparent only with the env-2 probe. (B) RT-PCR cloning mapped the 1.2-kb transcript (tr-env) as a prematurely polyadenylated mRNA terminating at position 6301. (C) Transfection of plasmid pCMV3JS21ΔGPΔStuI that expresses only the 1.2-kb tr-env mRNA in 293T cells and Northern blotting.