Table 1.
State-of-the-art of hybrid vector technologies. The mentioned delivery systems are helper-dependent adenovirus (HDAdV), herpes simplex virus type 1 (HSV-1), retrovirus (RV), and integrase defective lentivirus (IDLV). Integration machineries used in combination with these delivery vehicles are Sleeping Beauty (SB) transposase, bacteriophage integrase PhiC31, retrotransposon, adeno-associated virus (AAV; AAV-ITR or AAV-Rep protein-mediated), retrovirus (RV; LTR-mediated or retrovirus integrase mediated), zinc-finger nuclease (ZFN), and DNA-binding domain-retroviral integrase-fusion proteins (DBD-IN fusion proteins). (+) represents the existing combination of the respective integration machineries and the delivering vector. (−) represents non-existing viral hybrid vector systems.
| Integration machinery | SB | PhiC31 | Retrotransposon | AAV | RV | ZFN | DBD-IN-fusion proteins |
|---|---|---|---|---|---|---|---|
| Delivering vehicle | |||||||
| HDAdV | + | + | + | + | + | − | − |
| HSV-1 | + | − | − | + | + | − | − |
| RV | − | − | − | − | / | − | + |
| IDLV | + | − | − | − | − | + | − |