Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Nov 10;1(3):895–919. doi: 10.3390/v1030895

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Figure 4. — Different subcellular localization of plus- and minus-stranded DI-72 RNA during replication in yeast. (A) Schematic presentation of subcellular localization of MS2/CP-YFP and CFP-p33 in yeast in the absence of viral RNA and (B) in the presence of DI-72(+)/MS2. Specific interaction between the MS2 CP and the MS2 CP recognition hairpin (present in six copies in DI-72(+)/MS2) should result in re-localization of MS2 CP as shown. (C) Co-localization of CFP-tagged p33 and the YFP-tagged MS2/CP bound to the DI-72(+)/MS2 RNA in yeast cells using epifluorescence microscopy. Note that DI-72(–)/MS2 RNA (bottom panel) contains the six copies of MS2 CP recognition hairpins in complementary orientation. Therefore, MS2/CP-YFP could only bind to the negative-stranded DI RNA, which is generated during the replication of DI-72(–)/MS2 RNA.

Figure 4. — Different subcellular localization of plus- and minus-stranded DI-72 RNA during replication in yeast. (A) Schematic presentation of subcellular localization of MS2/CP-YFP and CFP-p33 in yeast in the absence of viral RNA and (B) in the presence of DI-72(+)/MS2. Specific interaction between the MS2 CP and the MS2 CP recognition hairpin (present in six copies in DI-72(+)/MS2) should result in re-localization of MS2 CP as shown. (C) Co-localization of CFP-tagged p33 and the YFP-tagged MS2/CP bound to the DI-72(+)/MS2 RNA in yeast cells using epifluorescence microscopy. Note that DI-72(–)/MS2 RNA (bottom panel) contains the six copies of MS2 CP recognition hairpins in complementary orientation. Therefore, MS2/CP-YFP could only bind to the negative-stranded DI RNA, which is generated during the replication of DI-72(–)/MS2 RNA.