Figure 9.

(A) Effect of Heparin on Adenovirus Transduction Efficiency. Viruses were pre-incubated with heparin for 1 hour at 37 °C prior to addition to A549 cells. Virus binding was allowed to take place for 1 hour at 4 °C prior to the replacement of virus with culture medium. Values are representative of three separate experiments. Error bars represent the standard deviation of the data; (B) PEGylated Adenoviruses Enter Target Cells Partially Through Heparan Sulfate Glycosaminoglycans. Virus was pre-incubated with heparin (10 μg/ml) prior to addition to monolayers of A549 cells treated with both the anti-CAR antibody and a peptide which blocks integrin receptors (RmcB + RGD + Hep.). Percent transduction is the number of beta-galactosidase positive cells found in treated monolayers with respect to the number of positive cells found in monolayers that did not receive treatment prior to viral infection. Data are the average transduction efficiencies obtained from two separate experiments and error bars represent the standard deviation of the data.