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. 2010 Feb 17;2(2):655–675. doi: 10.3390/v2020655

Figure 5.

Figure 5.

Arthropod cells transfected with a NSs expression plasmid efficiently express NSs. A. A. albopictus cells were transfected with an empty vector (Mock), NSs expression plasmid (pIB-NSs), or GFP expression plasmid (pIB-GFP). Cells were grown at 28 °C for 36 h. B. A stable D. melanogaster cell line was developed using an NSs expression plasmid with a copper-inducible metallothionein promoter (pMT-NSs). Expression was induced with 1 mM CuSO4 and cells were then grown at 28 °C for 16 h. The NSs antibody complex was labeled with anti-mouse 488 (green) and the nuclei with DAPI (blue). Scale bars represent 20 μm.