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. 2010 Feb 17;2(2):655–675. doi: 10.3390/v2020655

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© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 6. — Reporter gene expression is inhibited by NSs in D. melanogaster cells. A. D. melanogaster cells were transfected with expression plasmids for either luciferase (pS2MT-LUC) or β-galactosidase (pS2MT-LacZ). In addition to the reporter plasmid, cells received either empty plasmid (pMT) or NSs expression plasmid (pMT-NSs). Expression of all genes was controlled by a copper-sensitive promoter (metallothionein). Reporter levels were measured at 24 h post-induction. The error bars reflect the standard errors of the mean for three independent experiments. B. D. melanogaster cells were transfected with pMT (Mock) or pS2MT-LUC and either empty plasmid (pMT) or NSs expression plasmid (pMT-NSs). RNA was harvested and both actin and luciferase mRNA levels were examined by semi-quantitative RT-PCR.