Table 1.
General features, database matches and functional assignments of coding sequences (cds) of the Bacillus phage TP21-L genome for which homologies (e-value < 0.01) to known proteins could be found. Molecular mass (MM) and isoelectric points (pI) of the gene products (gp) are indicated. An asterisk indicates transcription on the complementary strand.
| cds | start | stop | MM (kDa) | pI | amino acid homologies (best hits) and deduced putative function |
|---|---|---|---|---|---|
| 1 | 10 | 489 | 17.8 | 6.6 | Phage terminase small subunit (various Streptococcus, Bacillus, Clostridium and Enterococcus prophages) |
| 2 | 808 | 2127 | 51.8 | 8.4 | Putative large terminase subunit (Staphylococcus phage CNPH82) |
| 3 | 2299 | 3771 | 56.8 | 5.0 | SPP1 family phage portal protein; ORF003 Staphylococcus phage 187 |
| 4 | 3775 | 4800 | 39.9 | 9.4 | Putative head protein (Clostridium phage phi CD119); SPP1 family phage head morphogenesis protein |
| 7 | 5798 | 6634 | 30.8 | 5.9 | Putative major head protein (various Clostridium prophages) |
| 9 | 7040 | 7372 | 12.4 | 9.0 | Putative phage head-tail adaptor (Bacillus prophages) |
| 10 | 7372 | 7788 | 15.4 | 10.7 | Phage protein, HK97 gp10 family (Bacillus cereus NVH0597-99 and others) |
| 15 | 9651 | 12494 | 99.9 | 5.3 | Phage tail tape measure protein, TP901 (Bacillus prophages) |
| 16 | 12506 | 14020 | 57.8 | 6.0 | Phage putative tail component (Bacillus cereus subsp. cytotoxis NVH 391–98); phage tail fiber protein (Bacillus phage Gamma) |
| 17 | 14024 | 19144 | 192.6 | 6.3 | Phage minor structural protein (Bacillus prophages) |
| 19 | 19500 | 20291 | 29.7 | 8.9 | Lysin (Bacillus phage IEBH); prophage LambdaBa01, N-acetylmuramoyl-L-alanine amidase |
| 20* | 20372 | 21754 | 53.7 | 8.9 | Recombinase (Bacillus and Paenibacillus prophages) |
| 21* | 21754 | 22386 | 23.9 | 8.9 | Transcriptional regulator (Bacillus thuringiensis serovar monterrey BGSC 4AJ1) |
| 22 | 22645 | 22893 | 9.8 | 9.4 | Cro-like protein, phage associated (Lactobacillus phage Sal2) |
| 32 | 26585 | 27400 | 32.5 | 8.9 | ORF016 (Staphylococcus phage 42E); primosome, DnaD subunit (Geobacillus sp.) |
| 34 | 27570 | 28313 | 29.2 | 9.2 | Rha family regulatory protein (Bacillus weihenstephanensis KBAB4); phage regulatory protein, Rha family (Paenibacillus sp. JDR-2) |
| 40 | 30404 | 31744 | 51.3 | 6.1 | Replicative DNA helicase (Bacillus thuringiensis serovar tochigiensis BGSC 4Y1) |