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. 2001 Apr 3;98(8):4461–4465. doi: 10.1073/pnas.071054198

Figure 6.

Figure 6

Interaction of IRAK with TRAF6. IRAK-null I1A cells transfected with IRAK deletion mutants were either untreated (−) or treated with 100 units/ml IL-1 for 15 min (+). (A) Extracts from cells transfected with dDD, dUD, dKD, dC1, and dC2; DD + UD + C1 cells were immunoprecipitated with anti-IRAK and probed with anti-TRAF6 and anti-IRAK. (B) Anti-IRAK antibody (Santa Cruz Biotechnology; recognizes 440–712 residues of IRAK) does not immunoprecipitate dC1C2 well, although it does recognize dC1C2 on a Western blot (Fig. 4). Therefore, dC1C2 and a few other IRAK deletion mutants (dUD, dC1, and dC2) were immunoprecipitated with anti-TRAF6 and probed with anti-IRAK and anti-TRAF6. Wild-type 293 cells (WT) were used as a positive control.