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. 2001 Apr 10;98(8):4466–4471. doi: 10.1073/pnas.081070898

Figure 5.

Figure 5

Clearance of cholesterol in CT60 CHO cells by NPC1-GFP restores late endosomal tubular trafficking. (A–C) CT60 CHO cells transfected with NPC1-GFP (48 h) but not yet cleared of lysosomal cholesterol (A). In cholesterol-laden cells NPC1-GFP (B) locates to the surface of lysosomes (B and C) and in long nonmobile late endosomal tubules (C). (D and E) CT60 CHO cells transfected with NPC1-GFP (72 h) and cleared of cholesterol. Cholesterol clearance (D) reestablishes late endosomal tubular trafficking (E). All images are confocal. (Bars = 10 μm.) View nonmobile late endosomal tubules containing NPC-1 GFP or rhodamine-dextran in Movies 3 and 4.