Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Feb 10;12(3):1333–1347. doi: 10.1093/nar/12.3.1333

Chromosomal arrangement of the two main rDNA size classes of Ascaris lumbricoides.

E Back, H Felder, F Müller, H Tobler
PMCID: PMC318580  PMID: 6701086

Abstract

The two main rDNA size classes of 8.8 kb and 8.4 kb length from Ascaris lumbricoides, which comprise more than 95% of the total nuclear rDNA, are arranged as internally homogeneous clusters and are not intermingled with each other. The rRNA genes are present on a single autosomal locus as could be demonstrated by in situ hybridization of fluorochrome-labelled 18S and 26S rRNA to metaphase chromosomes of spermatocytes I. The quantitative ratio of the two rDNA size classes varies largely between individuals being on the average roughly 10:1 in the investigated wild population of A. lumbricoides. However, there are no differences in the hybridization pattern of the two rDNAs in the DNA eliminating nematode A. lumbricoides between germ line and somatic cells in any of the individuals that have been analyzed.

Full text

PDF
1333

Images in this article

1337Image
on p.1337
1339Image
on p.1339
1340Image
on p.1340
1342Image
on p.1342
1343Image
on p.1343

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauman J. G., Wiegant J., Borst P., van Duijn P. A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochromelabelled RNA. Exp Cell Res. 1980 Aug;128(2):485–490. doi: 10.1016/0014-4827(80)90087-7. [DOI] [PubMed] [Google Scholar]
  2. Bauman J. G., Wiegant J., Van Duijn P., Lubsen N. H., Sondermeijer P. J., Hennig W., Kubli E. Rapid and high resolution detection of in situ hybridisation to polytene chromosomes using fluorochrome-labeled RNA. Chromosoma. 1981;84(1):1–18. doi: 10.1007/BF00293359. [DOI] [PubMed] [Google Scholar]
  3. Beckingham K. The ribosomal DNA of Calliphora erythrocephala. The cistron classes of total genomic DNA. J Mol Biol. 1981 Jun 25;149(2):141–169. doi: 10.1016/0022-2836(81)90296-5. [DOI] [PubMed] [Google Scholar]
  4. Brahic M., Haase A. T. Detection of viral sequences of low reiteration frequency by in situ hybridization. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6125–6129. doi: 10.1073/pnas.75.12.6125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldstein P., Moens P. B. Karyotype analysis of Ascaris lumbricoides var. suum. Male and female pachytene nuclei by 3-D reconstruction from electron microscopy of serial sections. Chromosoma. 1976 Oct 28;58(2):101–111. doi: 10.1007/BF00701353. [DOI] [PubMed] [Google Scholar]
  6. Goldstein P., Straus N. A. Molecular characterization of Ascaris suum DNA and of chromatin diminution. Exp Cell Res. 1978 Oct 15;116(2):462–466. doi: 10.1016/0014-4827(78)90471-8. [DOI] [PubMed] [Google Scholar]
  7. Goodpasture C., Bloom S. E. Visualization of nucleolar organizer regions im mammalian chromosomes using silver staining. Chromosoma. 1975 Nov 20;53(1):37–50. doi: 10.1007/BF00329389. [DOI] [PubMed] [Google Scholar]
  8. Hawley R. S., Tartof K. D. The ribosomal DNA of Drosophila melanogaster is organized differently from that of Drosophila hydei. J Mol Biol. 1983 Jan 25;163(3):499–503. doi: 10.1016/0022-2836(83)90071-2. [DOI] [PubMed] [Google Scholar]
  9. Junakovic N., Poretti A., Amaldi F., Buongiorno-Nardelli M. Differently sized rDNA repeating units of Xenopus laevis are arranged as internally homogeneous clusters along the nucleolar organizer. Nucleic Acids Res. 1978 Apr;5(4):1335–1343. doi: 10.1093/nar/5.4.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moritz K. B., Roth G. E. Complexity of germline and somatic DNA in Ascaris. Nature. 1976 Jan 1;259(5538):55–57. doi: 10.1038/259055a0. [DOI] [PubMed] [Google Scholar]
  11. Mutafova T. Morphology and behaviour of sex chromosomes during meiosis in Ascaris suum. Z Parasitenkd. 1975 Jul 16;46(4):291–295. doi: 10.1007/BF00418522. [DOI] [PubMed] [Google Scholar]
  12. Müller F., Walker P., Aeby P., Neuhaus H., Felder H., Back E., Tobler H. Nucleotide sequence of satellite DNA contained in the eliminated genome of Ascaris lumbricoides. Nucleic Acids Res. 1982 Dec 11;10(23):7493–7510. doi: 10.1093/nar/10.23.7493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pellegrini M., Manning J., Davidson N. Sequence arrangement of the rDNA of Drosophila melanogaster. Cell. 1977 Feb;10(2):213–214. doi: 10.1016/0092-8674(77)90215-x. [DOI] [PubMed] [Google Scholar]
  14. Petes T. D. Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes. Cell. 1980 Mar;19(3):765–774. doi: 10.1016/s0092-8674(80)80052-3. [DOI] [PubMed] [Google Scholar]
  15. Renkawitz-Pohl R., Glätzer K. H., Kunz W. Ribosomal RNA genes with an intervening sequence are clustered within the X chromosomal ribosomal DNA of Drosophila hydei. J Mol Biol. 1981 May 5;148(1):95–101. doi: 10.1016/0022-2836(81)90237-0. [DOI] [PubMed] [Google Scholar]
  16. Smith G. P. Evolution of repeated DNA sequences by unequal crossover. Science. 1976 Feb 13;191(4227):528–535. doi: 10.1126/science.1251186. [DOI] [PubMed] [Google Scholar]
  17. Smith G. P. Unequal crossover and the evolution of multigene families. Cold Spring Harb Symp Quant Biol. 1974;38:507–513. doi: 10.1101/sqb.1974.038.01.055. [DOI] [PubMed] [Google Scholar]
  18. Szostak J. W., Wu R. Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae. Nature. 1980 Apr 3;284(5755):426–430. doi: 10.1038/284426a0. [DOI] [PubMed] [Google Scholar]
  19. Tartof K. D., Dawid I. G. Similarities and differences in the structure of X and Y chromosome rRNA genes of Drosophila. Nature. 1976 Sep 2;263(5572):27–30. doi: 10.1038/263027a0. [DOI] [PubMed] [Google Scholar]
  20. Tartof K. D. Unequal mitotic sister chromatid exchange and disproportionate replication as mechanisms regulating ribosomal RNA gene redundancy. Cold Spring Harb Symp Quant Biol. 1974;38:491–500. doi: 10.1101/sqb.1974.038.01.053. [DOI] [PubMed] [Google Scholar]
  21. Tobler H., Smith K. D., Ursprung H. Molecular aspects of chromatin elimination in Ascaris lumbricoides. Dev Biol. 1972 Feb;27(2):190–203. doi: 10.1016/0012-1606(72)90097-8. [DOI] [PubMed] [Google Scholar]
  22. Tobler H., Zulauf E., Kuhn O. Ribosomal RNA genes in germ line and somatic cells of Ascaris lumbricoides. Dev Biol. 1974 Dec;41(2):218–223. doi: 10.1016/0012-1606(74)90300-5. [DOI] [PubMed] [Google Scholar]
  23. Wellauer P. K., Dawid I. B. The structural organization of ribosomal DNA in Drosophila melanogaster. Cell. 1977 Feb;10(2):193–212. doi: 10.1016/0092-8674(77)90214-8. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES