Table 1. Primers used for semiquantitative real-time polymerase chain reaction analysis of β-actin, GFAP, CD11b, CD68, TNF, IFNβ, IL-6, and IL-10 transcript levels.
| cDNA | Sequence (5′ to 3′) |
|---|---|
| β-Actin | GCCCTGAGGCTCTTTTCCAG (fdwa) TGCCACAGGATTCCATACCC (revb) |
| GFAP | GAAAACCGCATCACCATTCC (fdwa) TCGGATCTGGAGGTTGGAGA (revb) |
| CD11b | GAGCAGCACTGAGATCCTGTTTAA (fdwa) ATACGACTCCTGCCCTGGAA (revb) |
| CD68 | GGATTGGATTGAGGAAGGAACTG (fdwa) GCCGCATGGCAGAGATG (revb) |
| TNF | ATGCTGGGACAGTGACCTGG (fdwa) CCTTGATGGTGGTGCATGAG (revb) |
| IFNβ | CGGACTTCAAGATCCCTATGGA (fdwa) TGGCAAAGGCAGTGTAACTCTTC (revb) |
| IL-6 | CCACGGCCTTCCCTACTTC (fdwa) TTGGGAGTGGTATCCTCTGTGA (revb) |
| IL-10 | CCTGGTAGAAGTGATGCCCC (fdwa) TCCTTGATTTCTGGGCCATG (revb) |
GFAP, glial fibrillary acidic protein; IFN, interferon; IL, interleukin; TNF, tumor necrosis factor.
a
Forward.
b
Reverse.