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ECM and EGF stimulate tyrosine phosphorylation of the EGFR in HEK293 cells. HEK293 cell lysates were immunoprecipitated with EGFR polyclonal antibody, separated on 10% polyacrylamide gels and immunoblotted with mAb PY99 (P-tyr) or EGFR polyclonal Ab. Immunoblots were quantified densitometrically and expressed graphically (Lower). Data were normalized to the amount of EGFR immunoreactivity in precipitates from polyL-lysine-attached cells and expressed as means ± SEM (n = 3).
