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. 2001 Apr 3;98(8):4472–4477. doi: 10.1073/pnas.081069098

Figure 2.

Figure 2

ECM and EGF stimulate phosphorylation of MAPK and PKB proteins in HEK293 cells. Serum-starved HEK293 cells were either held in suspension or replated for 3 h on tissue culture plastic coated with polyL-lysine (10 μg/ml), fibronectin (10 μg/ml), or laminin (10 μg/ml). Cells were then stimulated with EGF (10 ng/ml) or pervanadate (1 μM) for 1 h, as indicated. Aliquots of cell lysate (30 μg) were then separated on 10% polyacrylamide gels and immunoblotted with the indicated antibodies. Densitometric values from immunoblots are shown (Lower) and are expressed as a percentage of the values from pervanadate-treated cells (mean ± SEM for three observations).