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mRPN1 down-regulation induced with a dsRNA construct induces accumulation of pre-gRNAs. (A) qPCR of mRPN1, REN, and mitochondrial RNA precursors at day 3 of induction. (B) Minicircles fragments (g1–g4) with two or three gRNAs and maxicircle fragments (m1–m4) with gene clusters from both strands (dashed lines). We confirmed the size and sequence of all amplicons. The assays were normalized as in Figure 5.
