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. 2011 Oct;17(10):1821–1830. doi: 10.1261/rna.2815911

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FIGURE 8. — mRPN1 nuclease-resistant associations, and yeast two-hybrid analysis. Western blots of endogenous mRPN1 and MP42 (KREPA3), which is a core subunit of RECC at about 20 S, in glycerol gradients of mitochondrial extracts, either untreated (A) or treated (B) with RNases A, T1, and V1 and micrococcal nuclease (nuclease treat.). Narrow slices of the same blot were used for MP42, which migrates just below mRPN1. Fraction 1 is the top of the gradient with sedimentation standards indicated. (C) Protein factors identified in nuclease-resistant TAP-purified mRPN1. (D) Reciprocal purifications of tagged RGG2 and tagged 4160. TEV eluates examined in Western blots with mRPN1 and anti-CBP antibodies. (E) Cartoon indicating a direct interaction of mRPN1 with RGG2 in a yeast two-hybrid analysis (Supplemental Fig. S5). The study did not show mRPN1 interaction with the other two protein fusions.