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. 2011 Oct;17(10):1831–1845. doi: 10.1261/rna.2523911

FIGURE 1.

FIGURE 1.

Argonaute 2 is enriched at and around lipid droplets (LDs) in Huh7 cells with full-length HCV replicon. (I) Schematic of genotype 1b autonomously replicating full-length HCV replicon stably expressed in Huh7 cells. (II) Schematic of subgenomic pFK-1398neo/luc/NS3-3′/5.1 HCV replicon. (A) Ago2 does not localize at lipid droplets in native Huh7 cells (enlargement 1). (B) Ago2 is enriched in foci located at and around LDs in Huh7 HCV cells with the full-length HCV replicon (enlargement 2). (C) Nonstructural viral protein NS5A localizes at and around LDs (enlargement 3). (A–C) P-body protein Dcp1a, unlike Ago2, is always seen separate from LDs. (D) Western blot analysis of the lipid droplet fraction from HCV replicating cells confirms that Ago2 associates with LDs. LD protein ADRP was used as LD marker. Whole cell lysates and LD fraction were also probed with cytoplasmic protein γ-Tubulin to verify purity of LD separation. Scale bar corresponds to 20 μm.