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Argonaute 2 foci associate with miRNA-122 and dsRNA in Huh7 cells with replicating HCV RNA. (A) In Huh7 cells with full-length HCV replicon, miR-122 and Ago2 distribute into joint spots localized in the perinuclear area. (B) P-bodies (visualized using Dcp1a protein) and miRNA-122 spots are seen separated. (C) dsRNA (visualized using K1 antibody) shows punctate staining and exhibits foci overlapping with Ago2 foci. Scale bars correspond to 20 μm.
