FIGURE 4.

Impact of substituting nucleotide 1U of the minigenome template on RSV RNA replication. Minigenome replication was reconstituted in HEp-2 cells simultaneously infected with MVA-T7, and the resulting RNA was analyzed. Panels are as described for Figure 2, except that panel Cii shows quantitation of RNA initiated at positions +1 and +3; each value was calculated relative to the wt +1 initiation value, and each error bar represents range of the mean of two independent primer extension analyses.