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. 2011 Aug 23;105(7):1002–1011. doi: 10.1038/bjc.2011.303

Figure 1.

Figure 1

Angiogenesis modulation by CD9P-1 expression. (A) Human EC were incubated with culture medium alone (control), or supplemented with either VEGF (50 ng ml–1), or TNFα at antiangiogenic concentration (50 ng ml–1), for 48 h with reload of factors at 24 h. (B) Gene profiling of the differentially regulated genes in the above conditions. The cDNA of interest marked with an arrow, was identified by sequencing as the CD9-P1 gene. (C) Representative image of WB of BAEC harbouring the empty (lane 1) or CD9-P1 antisense transcript coding pci-neovector (lane 2) using 229T mAb. (D) Representative images of in vitro angiogenesis assay with BAEC harbouring the empty or CD9P-1 antisense transcript coding pci-neovector.