Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Sep 6;105(7):953–960. doi: 10.1038/bjc.2011.349

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2011 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Hypoxia-inducible factor-1α (HIF-1α) protein level and HIF1 activity are upregulated in breast cancer cells by E2 stimulation. (A) Steroid-deprived MCF-7, T47D and MDA MB-231 cells were stimulated with E2 (10 nM) at the indicated time points. Whole-cell lysates were prepared and analysed by western blotting using antibodies to HIF-1α. Expression of HIF-1α was normalised to actin. (B) Breast cancer lines were co-transfected with HRE-pGL3 promoter luciferase reporter system and control vector encoding for the Renilla luciferase gene under the control of the CMV promoter. Transfected cells were steroid starved for 48 h and incubated with E2 (10 nM) at indicated time points and Luciferase activity was measured and normalised relative to renilla luciferase units using Dual Luciferase reporter system. Results shown represent the mean±s.d. of three independent experiments. (C) Expression of HIF1 downstream target genes was analysed by western blotting. Culture condition and E2 stimulation are similar to (A). The VEGF and GLUT-1 levels were normalised to actin.