Figure 2.

Aspm knockdown perturbs neuronal migration at late stages of corticogenesis. (A) Images of E19 cortices electroporated at E15 with control (top panels) or shASPM (middle and bottom panels) and GFP expression plasmids. Images were stained for GFP (left) and either Hoechst (middle) or FoxP2 (right). Dashed lines (middle) represent the CP/IZ (top) and IZ/ SVZ (bottom) borders. Bar, 100 μm. (B) Distribution of electroporated cells across cortical zones at E19. (C) Distribution of electroporated cells within the VZ and SVZ at E19. Upward-facing error bars and black stars denote significant differences in fraction of SVZ cells. Downward-facing error bars and red stars denote significant differences in fraction of VZ cells. (D) Percent of electroporated cells that overlap with FoxP2 staining in slices at E19.