Figure 3.

Expression of stabilized β-catenin rescues defects caused by Aspm knockdown. (A) Fold Wnt-mediated luciferase expression over control (Ctl, non-Wnt-containing medium) in P19 cells transfected with a combination of control (Ctl) or ASPM (shA) small hairpin expression constructs and vector (vector) or stabilized β-catenin (S37A mutant, βcat) expression constructs following exposure to Wnt-containing medium (Wnt). (B) Images of E16 cortices after electroporation at E13 with either control or shASPM, pCAGIG vector or pCAG-β-catenin, and GFP expression plasmids. (Left panels) GFP alone. (Right panels) GFP and Hoechst. Dashed lines in the right panels indicate the CP/IZ border (top) and IZ/SVZ border (bottom). Bar, 50 μm. (C) Distribution of electroporated cells across cortical zones at E16. (D) Fraction of GFP⁺ cells found in the upper half of the CP. (E) Images of E19 cortices showing the distribution of GFP⁺ cells following electroporation of control or shASPM and either pNeuroD vector or pNeuroD-β-catenin. (Left panels) GFP alone. (Right panels) GFP and Hoechst stain. Bar, 100 μm. (F) Distribution of electroporated cells across cortical zones at E19.