Figure 3.

The Mid and Q-rich regions define the CUP effector domain. (A) Schematic representation of CUP deletion mutants used in this study and a summary of their activities, as described in Figure 2A. (B–D) The activities of CUP deletion mutants relative to wild type were tested using the F-Luc-5BoxB reporter as described in Figure 1. (E) The interaction between HA-tagged wild-type or mutant CUP protein and endogenous eIF4E was analyzed by coimmunoprecipitation as described in Figure 2B. (F) Wild-type and mutant CUP proteins were expressed at comparable levels. R-Luc-V5 served as a transfection control.