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. 2011 Mar 20;1(4):521–530.

Figure 2.

Figure 2

Cell line-dependent chemosensitization by Caveolin-1 knockdown of human pancreatic tumor cells to Gemcitabine. A, Confirmatory Western blot analysis of siRNA-mediated knockdown of Caveolin-1 in human pancreatic cancer cell lines MiaPaCa2 and Panc1 with two specific siRNAs directed against human Caveolin-1 (Cav-1 siRNA 1, 5'-GCUUCCUGAUUGAGAUUCAtt-3' [47]); Cav-1 siRNA 2, 5'-UGUGAUUGCAGAACCAGAAtt-3' [69]) (negative control siRNA #1 (Applied Biosystems, Darmstadt, Germany), contr. siRNA). B, For analysis of clonogenic survival, MiaPaCa2 or Panc1 cells were transfected with Caveolin-1 specific siRNA (Cav-1 siRNA 1/2) or control siRNA (contr. siRNA), plated as single cells on 6-well plates (BD, Heidelberg, Germany) and treated for 24 h with 250 nM Gemcitabine (Lilly Deutschland, Bad Homburg, Germany) 48 h after siRNA transfection. Colonies were counted microscopically after 9 days (MiaPaCa2) or 12 days (Panc1). Results are means ± s.d. (n = 3). Student's t-test compared Cav-1 siRNA transfected cells to siRNA controls. *P < 0.05.