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. Author manuscript; available in PMC: 2011 Oct 4.
Published in final edited form as: Mol Cancer Ther. 2009 Jun 9;8(6):1579–1588. doi: 10.1158/1535-7163.MCT-09-0013

Figure 6. Comparison of the heritability of gene expression upon treatment of MCF7 cells with either 5-Aza-CdR or DZNep.

Figure 6

MCF7 cells were treated with either 1 μM 5-Aza-CdR or 10 μM DZNep for 24 h. Cells were harvested for RNA extraction or for cellular extracts every day for 14 days beginning 24 hr after drug removal (day 1). A) Real time qPCR showing the heritability of KRT7 P1 transcript and KRT7 P2 transcript following drug treatments. B) Western blot showing how the levels of EZH2 change upon drug removal. Bots were analyzed using Fluor-S Multi-ImagerMAX instrument (Bio-Rad, Hercules, CA). C) Genes that were upregulated more than 2-fold by either drug treatment were determined by Illumina microarray. The graph shows the number of genes upregulated on day 1 and day 7 by the indicated drug treatments.