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. 2001 Mar 27;98(8):4486–4491. doi: 10.1073/pnas.081075798

Figure 2.

Figure 2

Localization of RK2-lacO (pZZ6) and pUC19-lacO (pAFS52) plasmids by tagging with GFP-LacI at 23°C. GFP-LacI was expressed from plasmid pGAP60 by induction with 0.2% l-arabinose, as described in Materials and Methods. Cell membranes were stained with FM 4–64 (red), DNA was stained with DAPI (blue), and GFP-tagged plasmids appear as green foci. (A–C) Strain JP872 (containing RK2-lacO) grown in M63/glucose. (D–F) Strain JP873 (containing RK2) grown in M63/glucose. (G–I) Strain JP872 (containing RK2-lacO) grown in LB/glucose, showing cells containing one (i), two (iii), three (v), four (iv), five (ii), and six (vi) foci. (J–L) Strain JP839 containing the pUC-lacO derivative pAFS52 grown in LB/glucose, showing cells containing one (i) and two (ii) foci, a cell nearly finished dividing (iii), and cells with multiple foci (iv). (Bars = 1 μm.)