Table II. Compilation of proteomics results from heat stress experiments performed with other plant species focusing on consensus and contradictory expression profiles. From the study of Ferreira et al. (4) only changes after 6 h of heat stress were considered. (*) Protein data from this study. The asterisk is underlined and in boldface when the protein data positively correlated with qRT-PCR data.
| Protein name | Functional category | References up | References down |
|---|---|---|---|
| sHsps | Small heat shock proteins | (5–8, 12–14), (*) | |
| Chloroplast chaperonins | HSP60s | (4, 5, 11), (*) | |
| Hsp70s | HSP70 and co-chaperones | (5, 6, 8, 9, 11), (*) | |
| Hsp90s and cochaperones | HSP90 and co-chaperones | (4, 6–8, 10, 11), (*) | |
| ClpB3/4, Hsp101 | HSP100 | (5, 8), (*) | |
| Peptidyl-prolyl isomerase AtFKBP65/ROF2 | FKBPs/Cyclophilins | (8), (*) | |
| Cyclophilin A-2 | - “ - | (9, 10) | |
| ATP synthase CF1α | Other proteins associated with light reactions | (4, 5) | |
| Glycine dehydrogenase | Proteins of photosynthetic and photorespiratory carbon metabolism | (5, 11) | |
| Glutathione-S-transferase | Redox regulation/Oxidative stress response | (5, 8, 10) | |
| Thiamine biosynthesis protein | Vitamin biosynthesis | (4, 5, 11), (*) | |
| GTP-binding nuclear protein Ran | Signal transduction | (4, 7, 9, 10) | |
| Granule-bound glycogen starch synthase | Starch synthesis & degardation | (6, 7) | |
| PS I-N | Proteins associated with PS I | (*) | (9) |
| Oxygen-evolving enhancer protein 2 | Proteins associated with PS II | (9) | (12), (*) |
| ATP synthase CF1β | Other proteins associated with light reactions | (11) | (11) (9) |
| Ribulose biphosphate carboxylase/oxygenase activase | Proteins of photosynthetic and photorespiratory carbon metabolism | (7, 11) | (4, 9), (*) |
| Phosphoribulokinase | - “ - | (11) | (5, 9), (*) |
| Glyceraldehyde 3-phosphate dehydrogenase | - “ - | (4, 10, 14) | (9, 11) |
| Chloroplast phosphoglycerate kinase | - “ - | (11) | (9) |
| Triosephosphate isomerase | - “ - | (11) | (12), (*) |
| Transketolase | - “ - | (5, 11) | (4), (*) |
| Chloroplast seduheptulose-1,7-bisphosphatase | - “ - | (11) | (9), (*) |
| NADP-malic enzyme | - “ - | (8) | (4) |
| Ketol-acid reductoisomerase, chloroplast precursor | Amino acid metabolism | (4) | (*) |
| Eucaryotic translation initiation factor 4E | Protein biosynthesis (not ribosome) | (7) | (*) |
| Eucaryotic translation initiation factor 5A | - “ - | (14) | (12) |
| Elongation factor TU | - “ - | (5, 7) | (*) |
| Dehydroascorbate reductase | Redox regulation/Oxidative stress response | (5) | (12) |
| Thioredoxin h | - “ - | (4, 5) | (*) |
| Superoxide dismutase | - “ - | (10) | (*) |
| Ascorbate peroxidase | - “ - | (11) | (*) |
| Pyruvate dehydrogenase, SU E1α | TCA | (5) | (*) |
| Nucleoside diphosphate kinase | Nucleotide metabolism | (5) | (10) |
| Adenosine diphosphate glucose pyrophosphatase | Sugar nucleotide metabolism | (9) | (12) |
| Cytosolic GAPDH | Glycolysis and gluconeogenesis | (9) | (9, 10) |
| Vacuolar ATP synthase | Pumps and transporters | (6, 7) | (*) |
| Glycine-rich RNA-binding protein (Grp1A) | RNA processing/binding | (14) | (*) |
| Plastidic ATP sulphurylase (APS) | S-assimilation | (4, 10) | (*)a |
| Biotin carboxylase | Glycerolipid metabolism | (4) | (*)a |
| Mitochondrial ATP synthase beta | Mitochondrial electron transport chain and ATP synthase | (4) | (5, 7, 11) |
| Mannitol dehydrogenase | Miscellaneous | (14) | (*) |
| Oxygen evolving complex protein 1 (PSBO) | Proteins associated with PS II | (9), (*) | |
| Ferredoxin-NADP(H) oxidoreductase | Other proteins associated with light reactions | (5, 10, 11), (*) | |
| RBCS2 | Proteins of photosynthetic and photorespiratory carbon metabolism | (5, 9) | |
| RbcL | - “ - | (9), (*) | |
| Fructose-bisphosphate aldolase | - “ - | (10), (*) | |
| Serine hydroxymethyltransferase | - “ - | (10), (*) | |
| Glycine/serine hydroxymethyltransferase | - “ - | (9), (*) | |
| Cytosolic glutamine synthetase | N-metabolism | (10), (*) | |
| Methionine synthase (METE) | Amino acid metabolism | (9, 10), (*) | |
| O-Acetylserine (thiol) lyase | - “ - | (4), (*) | |
| FtsH-like protein | Proteases/Peptidases | (4, 9) | |
| 2-phosphoglycerate dehydratase | Glycolysis and gluconeogenesis | (9), (*) | |
| Cytosolic Pyruvate kinase | - “ - | (10), (*) | |
| Malate dehydrogenase (MDH) | TCA | (9, 10) | |
| Cytoplasmic aconitate hydratase | Acetate metabolism /Glyoxylate cycle | (10), (*) | |
| Adenosylhomocysteinase | C1-metabolism | (4, 10), (*) | |
| S-adenosylmethionine synth(et)ase | - “ - | (7, 10, 11), (*) | |
| Glucose-1-phosphate adenyltransferase | Sugar nucleotide metabolism | (6, 7) |
a Two isoforms were detected in our study of which only one decreased significantly.