Fig. 3.

TAP tag purified T. brucei NADH:ubiquinone dehydrogenase complex. A, TAP100, TAP096, TAP055 and TAP054 complexes were purified and separated on a 10–14.5% polyacrylamide Tris-glycine gel and stained with Sypro Ruby. The listed proteins were identified in the bands shown with corresponding numbers. The sizes of the protein marker are indicated. The position of tagged proteins is indicated by arrowheads. B, TAP151 and TAP157 complexes were purified and separated on a 10–14.5% and labeled as in A.