Table II. Comparison of genomic and proteomic features from putative antigens associated with protection.
Characteristics of the global irradiated sporozoite list and Top 20 list are compared to known vaccine candidates, Pf genome (PlasmoDB) and the proteins represented on the Pf protein microarray. Attributes such as average (Ave) number of exons, gene (bp) and protein (aa) size, isoelectric point (pI), presence of features that are recognized by antibodies (transmembrane (TM) domains, signal peptide, PEXEL motif), were compared. Antigens used for comparison purposes are listed in supplementary Table S3.
| Top 20 list (n = 20) | Global list (n = 86) | Known sporozoite/liver stage antigens (n = 7) | Known blood stage antigens (n = 17) | Pf genome (n = 5479) | Pf Array proteins (n = 1200) | |
|---|---|---|---|---|---|---|
| Ave number of Exons | 2.3 | 3 | 1.6 | 1.9 | 2.5 | 2.3 |
| Ave Transcription Length (bp) | 5711 | 6909 | 2245 | 2274 | 2270 | 3272 |
| Ave protein length (aa) | 1902 | 2302 | 747 | 760 | 755 | 1087 |
| Ave pl value | 7.1 | 7.8 | 5.3 | 5.3 | 8 | 7.7 |
| Ave Mw (kDa) | 224 | 272 | 85 | 87 | 89 | 128 |
| Number with TM domains | 30% | 34% | 71% | 41% | 31% | 22% |
| Number with Signal peptide | 35% | 24% | 100% | 65% | 19% | 17% |
| Number with PEXEL motif | 45% | 53% | 71% | 23% | 27% | 30% |