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Associations between mad2, bub1, bubR1, and cdc20. (A) Western blots showing neither bub1 nor bubR1 coimmunoprecipitates with mad2 in extracts from mitotic cells (derived by shakeoff after 4-h blockage with nocodazole). Cdc20 coimmunoprecipitates with mad2 and weakly with bubR1 but not with bub1. (B) Western blot showing mad2 is entirely soluble in cell extracts. Supernatants (S) and pellets (P) were loaded on the basis of equivalent initial volumes.
