Table III. T-2 metabolism of CYP3A46. T-2 was incubated with HepG2-pcDNA, HepG2-CYP3A46 (active or inactive), and in the reactions containing ketoconazole (KTZ) or not for 3 h. T-2, HT-2, 3′-hydroxy-T-2, 3′-hydroxy-HT-2 were detected and analyzed by LC-MS/MS. Data are represented as the values of peak area. n.d., not detected.
| Analyte | T-2 |
|||||||
|---|---|---|---|---|---|---|---|---|
| Buffer | HepG2-pcDNA | HepG2-CYP3A46 | HepG2-CYP3A46 (inactive) | HepG2-pcDNA (0.5 μm KTZ) | HepG2-CYP3A46 (0.5 μm KTZ) | HepG2-pcDNA (5 μm KTZ) | HepG2-CYP3A46 (5 μm KTZ) | |
| T-2 | 403624 | 144905 | 166658 | 447384 | 175224 | 136566 | 199617 | 215262 |
| HT-2 | 96 | 30397 | 32197 | 219 | 38583 | 28014 | 33047 | 30092 |
| 3′-hydroxy-T-2 | n.d. | n.d. | 5296 | n.d. | 7 | 1419 | n.d. | 117 |
| 3′-hydroxy-HT-2 | 47 | 30 | 36 | 62 | 28 | 26 | 64 | 62 |