Table IV. HT-2 metabolism of CYP3A46. HT-2 was incubated with HepG2-pcDNA and HepG2-CYP3A46 (active or inactive) in the reactions containing ketoconazole (KTZ) or not for 3 h. HT-2 and 3′-hydroxy-HT-2 were detected and analyzed by LC-MS/MS. Data are represented as the values of peak area.
| Analyte | HT-2 |
|||||||
|---|---|---|---|---|---|---|---|---|
| Buffer | HepG2-pcDNA | HepG2-CYP3A46 | HepG2-CYP3A46 (inactive) | HepG2-pcDNA (0.5 μm KTZ) | HepG2-CYP3A46 (0.5 μm KTZ) | HepG2-pcDNA (5 μm KTZ) | HepG2-CYP3A46 (5 μm KTZ) | |
| HT-2 | 55877 | 37117 | 16367 | 65794 | 39667 | 49469 | 44060 | 58316 |
| 3′-hydroxy-HT-2 | 33 | 40 | 223 | 26 | 16 | 9 | 28 | 39 |