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. Author manuscript; available in PMC: 2011 Oct 4.
Published in final edited form as: Virology. 2010 May 8;404(1):5–20. doi: 10.1016/j.virol.2010.04.008

Figure 4. Replication capacity (RC) assay.

Figure 4

The RC assay was based on transfection of a 10kb chimeric molecular clone, which was constructed by using ligation-mediated recombination PCR. HIV-1 pNL4-3 was used as a backbone with insertion of the entire pol gene amplified from plasma-derived virus by RT-PCR.