YFP-linked Na,K-ATPase β1 and β2 subunits of the Na,K-ATPase colocalize with the endogenous Na,K-ATPase α1 subunit. Confocal microscopy images showing that YFP-β1 and YFP-β2 (green) colocalize with the endogenous Na,K-ATPase α1 subunit (red) in the lateral membranes of MDCK cells as detected by immunostaining of cells expressing YFP-β1 or YFP-β2 using monoclonal antibody against the Na,K-ATPase α1 subunit.