Table 2. Details of 10 microsatellite loci used to genotype guppies (Poecilia reticulata); number of alleles observed (A), observed (Ho) and expected heterozygosities (He) over the whole sample (389 individuals), including mean He within populations (Hs).

Locus	primer 5′ fluorescent label	allele size range (bp)	A	Ho	He	Hs	source species/variety	Reference/GenBank accession no.
AGAT10	NED	143–273	32	0.67	0.87	0.74	wild	Olendorf et al., 2004, BV097140
Pret-27	VIC	150–278	30	0.62	0.90	0.71	domestic	Watanabe et al., 2003, AB100321
Pret-32	PET	118–177	19	0.55	0.71	0.54	domestic	Watanabe et al., 2003, AB100325
Pre8	FAM	172–310	25	0.58	0.87	0.66	wild	Paterson et al., 2005, AY830940
Pca4	FAM	128–144	7	0.27	0.66	0.25	Poecilia catemaconis	AF170707
AGAT11	FAM	211–370	33	0.88	0.95	0.91	wild	Olendorf et al., 2004, BV097141
Pre15	VIC	176–314	36	0.88	0.95	0.89	wild	Paterson et al., 2005, AY830943
Pre26	PET	105–229	26	0.71	0.90	0.76	wild	Paterson et al., 2005, AY830946
Pre9	NED	159–252	24	0.74	0.80	0.75	wild	Paterson et al., 2005, AY830941
Pr92	VIC	150–170	11	0.35	0.54	0.37	wild	Becher et al., 2002, AF467906