Figure 4.

Coiled coils are not required for KPβ oligomerization. Regions of KANK1 implicated in KPβ multimerization were identified by their ability to bind to K1-739, after transfection in HEK-293T cells. KPβ or mutants were immunoprecipitated with an anti-PDGFRβ antibody, which recognizes an epitope indicated by the arrow. Co-immunoprecipitated KPβ and K1-739 were visualized by western blot with anti-FLAG antibodies. Only m6 and m12 did not co-precipitate with K1-739. Non-relevant lanes were removed for clarity.