Figure 4.

TLT-1 is enriched in HA⁺ samples in resting and activated platelets. (A) Domain organization of full-length TLT-1 and the TLT-1 splice variant depicting an Ig-like V-type domain (blue) with 4 cysteine residues followed by a single-pass transmembrane (TM) domain (green). The TLT-1 splice variant has a truncated cytoplasmic tail and does not contain the 3 intracellular cysteines or ITIM region.³² (B) Western blot analysis of TLT-1 using ABE-purified palmitoylated proteins from resting and thrombin-activated platelets. Arrow indicates full-length TLT-1; and arrowhead, a 25 kDa TLT-1 splice variant. Bands seen directly below full-length TLT-1 and bands seen below the TLT-1 splice variant are degradation products.³³ Also shown are 20 μg of the sample inputs that represent the protein sample added to the streptavidin agarose beads.