Table 1.
Novel candidates of the platelet palmitoylome
| Protein name | Gene name | Ratio | P |
|---|---|---|---|
| Apoptosis | |||
| Myc target protein 1 | MYCT1 | 3.10 | .039094 |
| Placenta-derived apoptotic factor | PDAF | 4.00 | .015866 |
| Cell growth and/or maintanence | |||
| Tetraspanin-33 | TSPAN33 | 6.25 | .002368 |
| Claudin-5 | CLDN5 | 3.33 | .030650 |
| Dynein heavy chain 1, axonemal | DNAH1 | 6.00 | .002867 |
| Metalloproteinase inhibitor 1 | TIMP1 | 3.29 | .032192 |
| Lysophosphatidylcholine acyltransferase 1 | LPCAT1 | 4.00 | .015866 |
| Transmembrane protein 97 | TMEM97 | 3.00 | .043487 |
| DNA repair | |||
| Histone H2A | H2AFX | 3.00 | .043487 |
| Immune response | |||
| CKLF-like MARVEL transmembrane domain-containing protein 3 | CMTM3 | 11.00 | .000116 |
| CKLF-like MARVEL transmembrane domain-containing protein 5 | CMTM5 | 7.43 | .001013 |
| Early activation antigen CD69 | CD69 | 5.00 | .006457 |
| Minor histocompatibility protein HA-1 | HMHA1 | 3.00 | .043487 |
| Ion transport | |||
| Calcium release-activated calcium channel protein 1 | ORAI1 | 5.00 | .006457 |
| Metabolism | |||
| [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase | BCKDK | 4.00 | .015866 |
| α-amylase 1 | AMY1A | 3.00 | .043487 |
| ATPase family AAA domain-containing protein 3A | ATAD3A | 3.00 | .043487 |
| Catechol-O-methyltransferase | COMT | 4.50 | .010000 |
| Dihydrolipoamide branched chain transacylase E2 | DBT2 | 8.00 | .000690 |
| Dolichyldiphosphatase 1 | DOLPP1 | 3.00 | .043487 |
| GPI ethanolamine phosphate transferase 1 | PIGN | 3.00 | .043487 |
| Probable cysteinyl-tRNA synthetase | CARS2 | 4.00 | .015866 |
| Protein disulfide-isomerase TMX3 | TMX3 | 15.00 | .000016 |
| Suppressor of Lec15 | MPDU1 | 3.00 | .043487 |
| Thioredoxin-related transmembrane protein 1 | TMX1 | 3.45 | .027084 |
| Thioredoxin-related transmembrane protein 4 | TMX4 | 6.00 | .002867 |
| V-type proton ATPase 116-kDa subunit α isoform 2 | ATP6V0A2 | 3.00 | .043487 |
| Protein metabolism | |||
| 26S proteasome non-ATPase regulatory subunit 11 | PSMD11 | 6.00 | .002867 |
| CAAX prenyl protease 1 homolog | ZMPSTE24 | 4.00 | .015866 |
| Copper chaperone for superoxide dismutase | CCS | 4.00 | .015866 |
| Cystatin-S | CST4 | 3.00 | .043487 |
| E3 ubiquitin-protein ligase MARCH2 | MARCH | 6.00 | .002867 |
| Eukaryotic translation elongation factor 1 ϵ-1 | EEF1E1 | 3.00 | .043487 |
| Eukaryotic translation initiation factor 5A-1 | EIF5A | 6.00 | .002867 |
| GrpE protein homolog 1 | GRPEL1 | 3.00 | .043487 |
| Leucyl/cystinyl aminopeptidase | LNPEP | 4.00 | .015866 |
| RING finger protein 11 | RNF11 | 3.50 | .025869 |
| Signal transduction | |||
| Atlastin-1 | ATL1 | 5.00 | .006457 |
| CKLF-like MARVEL transmembrane domain-containing protein 7 | CMTM7 | 4.00 | .015866 |
| Endothelial cells scavenger receptor | SCARF1 | 10.00 | .000202 |
| Filaggrin | FLG | 3.00 | .043487 |
| GTP-binding protein Rheb | RHEB | 4.00 | .015866 |
| Leucine-rich repeat-containing protein 32 | LRRC32 | 21.00 | .000002 |
| Metalloreductase STEAP3 | STEAP3 | 6.00 | .002867 |
| Nuclear receptor subfamily 4 group A member 2 | NR4A2 | 3.00 | .043487 |
| Platelet-derived endothelial cell growth factor | ECGF1 | 3.00 | .043487 |
| Rho-related GTP-binding protein RhoQ | RHOQ | 3.00 | .043487 |
| Tescalcin | TESC | 18.00 | .000005 |
| Tetraspanin-15 | TSPAN15 | 17.00 | .000007 |
| Transmembrane protein 11 | TMEM11 | 7.00 | .001366 |
| Transmembrane protein 50A | TMEM50A | 3.25 | .033406 |
| Transmembrane protein 55A | TMEM55A | 3.83 | .018614 |
| Transcription regulator activity | |||
| 39S ribosomal protein L12 | MRPL12 | 3.00 | .043487 |
| Oxidoreductase HTATIP2 | HTATIP2 | 3.00 | .043487 |
| Transport | |||
| BET1 homolog | BET1 | 10.00 | .000202 |
| BET1-like protein | BET1L | 11.00 | .000116 |
| Cholesteryl ester transfer protein | CETP | 4.00 | .015866 |
| MLN64 N-terminal domain homolog | STARD3NL | 4.00 | .015866 |
| Nonspecific lipid-transfer protein | SCP2 | 9.00 | .000366 |
| Proteolipid protein 2 | PLP2 | 3.00 | .043487 |
| SID1 transmembrane family member 1 | SIDT1 | 4.00 | .015866 |
| Sodium/hydrogen exchanger 9 | SLC9A9 | 3.67 | .021908 |
| Solute carrier family 43 member 3 | SLC43A3 | 5.00 | .006457 |
| Syntaxin-2 | STX2 | 3.00 | .043487 |
| Syntaxin-10 | STX10 | 4.00 | .015866 |
| Syntaxin-11 | STX11 | 6.18 | .002507 |
| Tandem C2 domains nuclear protein | TC2N | 42.00 | .000000 |
| Vesicle transport protein SFT2C | SFT2D3 | 6.00 | .002867 |
| Vesicle-associated membrane protein 5 | VAMP5 | 15.00 | .000016 |
| Unknown | |||
| Catechol-O-methyltransferase domain-containing protein 1 | COMTD1 | 3.50 | .025869 |
| CD99 antigen-like protein 2 | CD99L2 | 3.00 | .043487 |
| Chronic lymphocytic leukemia deletion region gene 6 protein | CLLD6 | 4.50 | .010000 |
| COMM domain-containing protein 9 | COMMD9 | 3.00 | .043487 |
| Cyclin-Y | CCNY | 9.00 | .000366 |
| Exocyst complex component 3-like protein 2 | EXOC3L2 | 4.00 | .015866 |
| Leptin receptor gene-related protein | LEPROT | 6.00 | .002867 |
| Lipoma HMGIC fusion partner | LHFP | 8.00 | .000690 |
| Lipoma HMGIC fusion partner-like 2 protein | LHFPL2 | 6.40 | .002115 |
| LITAF-like protein | CDIP | 6.00 | .002867 |
| Major facilitator superfamily domain-containing protein 6 | MFSD6 | 4.00 | .015866 |
| Malectin | MLEC | 4.90 | .007035 |
| Metallo-β-lactamase domain-containing protein 2 | MBLAC2 | 12.00 | .000068 |
| Mps one binder kinase activator-like 3 | MOBKL3 | 5.00 | .006457 |
| Oligosaccharyltransferase complex subunit OSTC | OSTC | 3.00 | .043487 |
| PDZK1-interacting protein 1 | PDZK1IP1 | 8.00 | .000690 |
| Prolactin-inducible protein | PIP | 4.00 | .015866 |
| Protein EFR3 homolog A | EFR3A | 9.67 | .000245 |
| Protein FAM78A | FAM78A | 3.00 | .043487 |
| Protein S100-A14 | S100A14 | 3.00 | .043487 |
| Putative uncharacterized protein C1orf150 | C1orf150 | 10.00 | .000202 |
| Putative uncharacterized protein C1orf95 | C1orf95 | 3.00 | .043487 |
| SEC6-like protein C14orf73 | C14orf73 | 24.00 | .000001 |
| Small VCP/p97-interacting protein | SVIP | 3.00 | .043487 |
| Tetraspanin-14 | TSPAN14 | 9.80 | .000227 |
| Transmembrane BAX inhibitor motif-containing protein 1 | TMBIM1 | 6.00 | .002867 |
| Transmembrane protein 222 | TMEM222 | 10.00 | .000202 |
| Transmembrane protein 50B | TMEM50B | 8.00 | .000690 |
| Transmembrane protein with metallophosphoesterase domain | TMPPE | 5.00 | .006457 |
| Uncharacterized protein C22orf25 | C22orf25 | 7.00 | .001366 |
| Uncharacterized protein KIAA2013 | KIAA2013 | 7.00 | .001366 |
| UPF0389 protein FAM162A | FAM162A | 8.50 | .000500 |
| UPF0598 protein C8orf82 | C8orf82 | 11.00 | .003588 |
| UPF0733 protein C2orf88 | C2orf88 | 8.00 | .000116 |
To date, these 103 proteins have not been shown to be palmitoylated and represent putative palmitoylated platelet proteins. Data are the result of 2 independent experiments, and proteins are listed according to the biologic process they mediate as defined by the HPRD. Also shown is the HA+/HA− ratio and corresponding P value.