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. 2011 Aug 12;286(40):34809–34819. doi: 10.1074/jbc.M111.281626

FIGURE 5.

FIGURE 5.

SBF and Rpd3(L) binding to HO URS2 is weak compared with binding at CLN2. A, strain DY12794 (GALp::CDC20 SWI4-Myc) was synchronized, and ChIP samples were taken at various times following release from CDC20 arrest. SBF binding to HO URS2 was measured with primers that amplify from −825 to −489 and binding to CLN2 with primers that amplify from −661 to −379. B, strain DY12247 (GALp::CDC20 SDS3-Myc) was synchronized, and ChIP samples were taken at various times following release from CDC20 arrest. SBF binding to HO URS2 was measured with primers that amplify from −825 to −489 and binding to CLN2 with primers that amplify from −661 to −379. Sds3 is a subunit specific to Rpd3(L). C, strains DY12247 (GALp::CDC20 SDS3-Myc) and DY12830 (GALp::CDC20 SDS3-Myc ash1 swi6) were synchronized, and ChIP samples were taken at various times following release from CDC20 arrest. Rpd3(L) binding to HO URS1 was measured with primers that amplify from −1429 to −1139. D, strains DY12247 (GALp::CDC20 SDS3-Myc) and DY12830 (GALp::CDC20 SDS3-Myc ash1 swi6) were synchronized, and ChIP samples were taken at various times following release from CDC20 arrest. Rpd3(L) binding to HO URS2 was measured with primers that amplify from −825 to −489.