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. 2011 Aug 12;286(40):34809–34819. doi: 10.1074/jbc.M111.281626

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Defect in HO expression caused by a taf1 mutation can be suppressed by whi5 or ash1. A, HO mRNA levels were measured by RT-qPCR from cells growing logarithmically at 33 °C: DY150, wild type; DY10598, taf1(ΔTAND); DY11348, taf1Δ YCp(TRP1)-TAF1; DY11349, taf1Δ YCp(TRP1)-TAF1(Y570N); DY12303, taf1Δ YCp(HIS3)-TAF1-HA; and DY12305, taf1Δ YCp(HIS3)-TAF1(N568Δ)-HA. B, ChIP experiments were performed from cells growing logarithmically at 33 °C: DY10204 (taf1Δ YCp(TRP1)-TAF1) and DY10366 (taf1Δ YCp(TRP1)-TAF1 ash1) as untagged controls. YCp(HIS3) plasmids expressing TAF1-HA, TAF1(T657K)-HA, or TAF1(N568Δ)-HA were shuffled into strains DY10204 (ASH1) and DY10366 (ash1), and ChIP experiments were performed with the HA-tagged TAF1 as the only copy in the cell. Taf1 binding to the HO TATA was measured with primers that amplify from −43 to +175. C, HO mRNA levels were measured by RT-qPCR from cells growing logarithmically at 33 °C: DY11348, taf1Δ YCp(TRP1)-TAF1; DY11574, taf1Δ YCp(TRP1)-TAF1 whi5; DY11349, taf1Δ YCp(TRP1)-TAF1(Y570N); and DY11575, taf1Δ YCp(TRP1)-TAF1(Y570N) whi5. D, HO mRNA levels were measured by RT-qPCR from cells growing logarithmically at 33 °C: DY11348, taf1Δ YCp(TRP1)-TAF1; DY11570, taf1Δ YCp(TRP1)-TAF1 ash1; DY11349, taf1Δ YCp(TRP1)-TAF1(Y570N); and DY11571, taf1Δ YCp(TRP1)-TAF1(Y570N) ash1.