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. 2011 Aug 10;286(40):34627–34634. doi: 10.1074/jbc.M111.274571

FIGURE 4.

FIGURE 4.

pH dependence of the NADH:decyl-ubiquinone (A) and NADPH:decyl-ubiquinone (B) oxidoreductase activity of complex I (squares) and the E183HF variant (circles). The activity was measured between pH 5.0 and 8.0. All activities were more than 95% sensitive to the addition of 10 μm piericidin A. The maximum activity of the NADH and NADPH oxidation was 2.8 and 0.6 μm·min−1·mg−1 for complex I, respectively, and 4.0 and 1.3 μm·min−1·mg−1 for the E183HF variant. The other variants showed a similar pH activity profile with a maximum NADH oxidation rate between pH 6.0. and 7.0 and a maximum NADPH oxidation rate between 6.0 and 6.5.