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Superoxide and H2O2 production by complex I and the E183HF variant measured as Amplex Red oxidation
Piericidin A is abbreviated as PicA; decyl-ubiquinone is abbreviated as Dec-Q.
| Sample | NAD(P)H-induced superoxide and H2O2 production |
||||
|---|---|---|---|---|---|
| None | +NADH | +NADPH | +NADPH, +PicA | +NADPH, −Dec-Q | |
| nmol·min−1·mg−1 | |||||
| Complex I | 0 | 17 ± 0.3 | 40 ± 0.9 | 75 ± 1.2 | 26 ± 0.7 |
| E183HF | 0 | 25 ± 0.4 | 29 ± 0.6 | 23 ± 0.6 | 11 ± 0.3 |
