FIGURE 8.

β-Catenin inhibition of plasma membrane resealing. ECV304 cells expressing GFP (A–B) or GFP-β-catenin (GFP-β-ctn) (C) were laser-injured in the absence (A) and presence (B and C) of Ca²⁺-containing PBS and FM4-64, a cell membrane-impermeable fluorescence dye. Time lapse images were recorded. Images shown were before (0 min) and after the injury (200 and 400 min). Stars, injury sites; triangles, uninjured control cells. D, quantification of FM4-64 dye uptake. ECV304 cells expressing β-catenin showed significantly more dye influx. *, p < 0.05, significant difference by repeated measures analysis of variance, compared with GFP-expressing ECV304 cells. Error bars, S.D.