. 2011 Aug 19;286(40):35275–35282. doi: 10.1074/jbc.M111.246439

TABLE 1.

Hsp90 binding to the transiently transfected AhR proteins

Binding to the indicated mutant or wild-type AhR was analyzed in triplicate using the co-immunoprecipitation/Western blot protocol described under “Experimental Procedures.” Values are expressed as a percent of the wtAhR and presented as the means ± S.D. of three replicate reactions adjusted for nonspecific binding reactions (IgM controls). Statistical comparisons were performed using the corresponding wtAhR controls, and only one representative wtAhR value is presented.

AhR mutation	Hsp90 binding
T276A	111 ± 8
K277A	105 ± 16
N278A	105 ± 15
F279A	105 ± 10
I280A	65 ± 16
F281A	5 ± 10^a
R282A	83 ± 19
T283A	−1 ± 10^a
K284A	90 ± 8
H285A	9 ± 6^a
K286A	89 ± 2
L287A	29 ± 7^a
D288A	13 ± 2^a
F289A	63 ± 4^a
T290A	73 ± 17
F281L	17 ± 8^a
T283E	20 ± 6^a
T283M	64 ± 16^a
H285F	123 ± 25
3M (F279T/I280E/R282I)	13 ± 6^a
A375L	64 ± 17^a
A375V	77 ± 18
wtAhR	100 ± 18

^a Statistically different from the wtAhR at p < 0.05 as determined by Student's t test.