FIGURE 4.

Paucity of α-syn inclusions in TH-positive neurons of the substantia nigra and projection in the striatum in E46K α-syn transgenic mice. A, D, and G, staining with anti-TH antibody (green); B, E, and H, pSer129 antibody (red) in the midbrain (A–F) or striatum (G–I) of a 22-month-old symptomatic M47 transgenic mouse. C, F, and I, overlay of the immune staining with Hoechst labeling (blue). The images demonstrate a paucity of α-syn inclusions in these TH-positive neurons and processes. Bar, 400 μm (A–C) and 200 μm (D–I).