FIGURE 6.
Decreased mitochondrial Bax levels and cell death by DJ-1 under UVB irradiation. A, H1299 cells transfected with si-NC or si-DJ-1 were treated with or without 80 mJ/cm2 UVB irradiation, and the cells were then subjected to the subcellular fractionation assay. The cytosolic and mitochondrial fractions were subjected to immunoblot analysis. B, cell lysates of H1299 cells transfected with si-NC or si-Bcl-XL were subjected to immunoblot analysis. C and D, H1299 cells transfected with si-NC, si-DJ-1, or along with si-Bcl-XL were treated with or without 80 mJ/cm2 UVB irradiation. Cell lysates were then subjected to immunoblot analysis (C) or the cells were analyzed with the MTT assay (D) (mean ± S.E., n = 3; *, p < 0.05; ns, no statistical significance by one-way ANOVA).