FIGURE 4.

Comparison of relative abundance estimated by high resolution MS and individual Western data for five LPXTG proteins identified with high coverage in proteomics: Lmo0130, Lmo0160, Lmo0610, Lmo0880, and InlA. Cell wall extracts were prepared from L. monocytogenes EGDe strain grown in minimal medium IMM to stationary phase. Note that for an equal volume of sample loaded in the gel (internal control represented by levels of Lmo0160 protein), distinct intensities are observed in the Western assays performed with the corresponding anti-Lmo0130, anti-Lmo0160, anti-Lmo0610, anti-Lmo0880, and anti-InlA antisera. Note the differences observed in comparative terms with the rest of the proteins for the case of Lmo0610, detected as a very abundant protein in the Western assay but with a lower coverage in proteomics. Sizes of the molecular weight markers used are also indicated.